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Reversible Ca2+ Switch of An Engineered Allosteric Antioxidant Selenoenzyme
Jan 6, 2015

Allosteric SelenoenzymeA Ca2+-responsive artificial selenoenzyme was constructed by computational design and engineering of recoverin with the active center of glutathione peroxidase (GPx). By combining the recognition capacity for the glutathione (GSH) substrate and the steric orientation of the catalytic selenium moiety, the engineered selenium-containing recoverin exhibits high GPx activity for the catalyzed reduction of H2O2 by glutathione (GSH). Moreover, the engineered selenoenzyme can be switched on/off by Ca2+-induced allosterism of the protein recoverin. This artificial selenoenzyme also displays excellent antioxidant ability when it was evaluated using a mitochondrial oxidative damage model, showing great potential for controlled catalysis in biomedical applications. This work was recently reported by Junqiu Liu’s group in Angewandte Chemie International Edition (Angew. Chem. Int. Ed.2014,53, 13536-13539)

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